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Rabbit anti-EDD1 Antibody
SKU: orb1525555
Description
Images & Validation
−Item 1 of 1
| Tested Applications | IP, WB |
|---|---|
| Dilution range | WB - 1:2,000 - 1:10,000; IP - 2 - 5 µg/mg lysate |
| Reactivity | Human, Mouse |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Immunogen | Between 2749 and C-terminus |
| Target | EDD1 |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | 2 - 8°C |
|---|---|
| Form/Appearance | Whole IgG |
| Buffer/Preservatives | Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide |
| Concentration | 1000 µg/ml |
| Disclaimer | For research use only |
Alternative Names
−HECT-type E3 ubiquitin transferase UBR5; DD5; progestin-induced protein; hyperplastic discs protein homolog; HYD; hHYD; EDD1; EDD; E3 ubiquitin-protein ligase, HECT domain-containing 1; E3 identified by differential display; E3 ubiquitin-protein ligase UBR5
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Detection of human and mouse EDD1 by western blot (h&m) and immunoprecipitation (h). Samples: Whole cell lysate from HeLa (H; 50 µg for WB; 1 mg for IP, 20% of IP loaded) , HEK293T (5, 15, and 50 µg for WB) and mouse NIH 3T3 (M; 50 µg) cells. Antibodies: Affinity purified rabbit anti-EDD1 antibody orb1525555 used for WB at 0.4 µg/ml (A) and 1 µg/ml (B) and used for IP at 6 µg/mg lysate. EDD1 was less efficiently immunoprecipitated by rabbit anti-EDD1 antibody, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).
Quick Database Links
UniProt Details
− No UniProt data available
NCBI Reference Sequences
−Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product| Protein | NP_056986.2 |
|---|
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Datasheet
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Protocol Information
WB
Western Blot (IB, immunoblot)
IP
Immunoprecipitation
Rabbit anti-EDD1 Antibody (orb1525555)
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