Rabbit anti-JARID1B Antibody

SKU: orb1527741

Description

JARID1B Antibody

Images & Validation

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Item 1 of 3

Tested Applications	IHC, IP, WB
Dilution range	WB - 1:2,000 - 1:10,000; IP - 2 - 10 µg/mg lysate; IHC - 1:500 - 1:2,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Reactivity	Human
Application Notes	Format: Whole IgG

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Immunogen	Between 1494 and C-terminus
Target	JARID1B
Conjugation	Unconjugated

Storage & Handling

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Storage	2 - 8°C
Form/Appearance	Whole IgG
Buffer/Preservatives	Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration	1000 µg/ml
Disclaimer	For research use only

Alternative Names

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PLU-1; retinoblastoma-binding protein 2 homolog 1; CT31; RBP2-H1; retinoblastoma-binding protein 2, homolog 1A; RBBP2H1A; putative DNA/chromatin binding motif; PUT1; protein phosphatase 1, regulatory subunit 98; PPP1R98; MRT65; lysine-specific demethylase 5B; lysine (K)-specific demethylase 5B; jumonji/ARID domain-containing protein 1B; jumonji, AT rich interactive domain 1B; histone demethylase JARID1B; cancer/testis antigen 31; JARID1B; PLU1

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Detection of human JARID1B by western blot. Samples: Whole cell lysate (50 µg) from HeLa, 293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-JARID1B antibody orb1527741 used for WB at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Detection of human JARID1B by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-JARID1B antibody orb1527741.

Detection of human JARID1B by immunohistochemistry.Sample: FFPE section of human prostate carcinoma. Antibody: Rabbit anti-JARID1B antibody (orb1527741) used at 1: 500 (2µg/ml). Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.

Quick Database Links

Gene Symbol

JARID1B

UniProt

Q9UGL1

RefSeq (Protein):NP_006609.3

UniProt Details

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No UniProt data available

NCBI Reference Sequences

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Associated Accession Numbers

Curated reference sequences for the gene transcript and protein product

Protein	NP_006609.3

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Protocol Information

Western Blot (IB, immunoblot)

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IHC

Immunohistochemistry

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Immunoprecipitation

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