Rabbit NFX1 Antibody

SKU: orb1525767

Description

NFX1 Antibody

Research Area

Cancer Biology, Immunology & Inflammation

Images & Validation

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Item 1 of 1

Tested Applications	IP, WB
Dilution Range	WB - 1:2,000 - 1:10,000; IP - 2 - 5 µg/mg lysate
Reactivity	Human, Mouse
Application Notes	Format: Whole IgG

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	Between 1070 and C-terminus
Target	NFX1
Purification	Antigen Affinity Purified
Conjugation	Unconjugated

Storage & Handling

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Storage	2 - 8°C
Form/Appearance	Liquid
Buffer/Preservatives	Tris-buffered Saline containing 0.1% rAlbumin and 0.09% Sodium Azide
Concentration	200 µg/ml
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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NFX2; nuclear transcription factor, X box-binding protein 1; TEG-42; Tex42; transcriptional repressor NF-X1

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Detection of human and mouse NFX1 by western blot (h&m) and immunoprecipitation (h). Samples: Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) , HEK293T (T; 50 µg) and mouse NIH 3T3 (M; 50 µg) cells. Antibodies: Affinity purified rabbit anti-NFX1 antibody orb1525767 used for WB at 0.04 µg/ml (A) and 0.4 µg/ml (B) and used for IP at 3 µg/mg lysate. NFX1 was also immunoprecipitated by rabbit anti-NFX1 antibodies, which recognize upstream epitopes. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 10 seconds (B).

Quick Database Links

Gene Symbol

NFX1

UniProt

Q12986

RefSeq (Protein):NP_002495.2

UniProt Details

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No UniProt data available

NCBI Reference Sequences

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Associated Accession Numbers

Curated reference sequences for the gene transcript and protein product

Protein	NP_002495.2

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Protocol Information

Western Blot (IB, immunoblot)

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Immunoprecipitation

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