Rabbit anti-RAD23B Antibody

SKU: orb1526861

Description

RAD23B Antibody

Images & Validation

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Item 1 of 4

Tested Applications	IHC, IP, WB
Dilution range	WB - 1:2,000 - 1:10,000; IP - 10 µg/mg lysate; IHC - 1:500 - 1:2,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Reactivity	Human, Mouse, Rat
Application Notes	Format: Whole IgG

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Immunogen	Between 359 and C-terminus
Target	RAD23B
Conjugation	Unconjugated

Storage & Handling

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Storage	2 - 8°C
Form/Appearance	Whole IgG
Buffer/Preservatives	Tris-buffered Saline containing 0.1% rAlbumin and 0.09% Sodium Azide
Concentration	200 µg/ml
Disclaimer	For research use only

Alternative Names

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XP-C repair complementing complex 58 kDa; XP-C repair complementing protein; HHR23B; XP-C repair-complementing complex 58 kDa protein; UV excision repair protein RAD23 homolog B; RAD23, yeast homolog of, B; HR23B; P58

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Detection of mouse RAD23B by western blot. Samples: Whole cell lysate (50 µg) from NIH 3T3, TCMK-1, 4T1, and CT26.WT cells. Antibodies: Affinity purified rabbit anti-RAD23B antibody orb1526861 used for WB at 0.2 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Detection of rat RAD23B by western blot. Samples: Whole cell lysate (50 µg) from C6 cells. Antibodies: Affinity purified rabbit anti-RAD23B antibody orb1526861 used for WB at 0.2 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Detection of human and mouse RAD23B by western blot (h&m) and immunoprecipitation (h). Samples: Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) and mouse NIH 3T3 (M; 50 µg) cells. Antibodies: Affinity purified rabbit anti-RAD23B antibody orb1526861 used for WB at 0.04 µg/ml (A) and 0.4 µg/ml (B) and used for IP at 10 µg/mg lysate. RAD23B was also immunoprecipitated by rabbit anti-RAD23B antibody, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 3 seconds (B).

Detection of human RAD23B by immunohistochemistry.Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-RAD23B (Cat. No. orb1526861) used at a dilution of 1: 1, 000 (0.2 µg/ml). Detection: DAB

Quick Database Links

Gene Symbol

RAD23B

UniProt

P54727

RefSeq (Protein):NP_002865.1

UniProt Details

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No UniProt data available

NCBI Reference Sequences

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Associated Accession Numbers

Curated reference sequences for the gene transcript and protein product

Protein	NP_002865.1

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Protocol Information

Western Blot (IB, immunoblot)

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IHC

Immunohistochemistry

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Immunoprecipitation

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