Rabbit RBM22 Antibody

SKU: orb1523927

Description

RBM22 Antibody

Images & Validation

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Item 1 of 3

Tested Applications	IP, WB
Dilution range	WB - 1:10,000 - 1:25,000; IP - 2 - 10 µg/mg lysate
Reactivity	Human, Mouse
Predicted Reactivity	Bovine, Frog, Gallus, Rat, Xenopus, Zebrafish
Application Notes	Format: Whole IgG

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	Between 175 and 225
Target	RBM22
Purification	Antigen Affinity Purified
Conjugation	Unconjugated

Storage & Handling

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Storage	2 - 8°C
Form/Appearance	Liquid
Buffer/Preservatives	Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration	1000 µg/ml
Disclaimer	For research use only

Alternative Names

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Cwc2; fSAP47; functional spliceosome-associated protein 47; pre-mRNA-splicing factor RBM22; RNA-binding motif protein 22; ZC3H16; zinc finger CCCH domain-containing protein 16

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Detection of mouse RBM22 by western blot. Samples: Whole cell lysate (10 µg) from NIH 3T3, CT26, CH27, TCMK-1, and BW5147.3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-RBM22 antibody (orb1523927) used for WB at 0.04 µg/ml. Detection: Chemiluminescence with an exposure time of 1 second.

Detection of human RBM22 by western blot. Samples: Whole cell lysate (10 µg) from HEK293T, HeLa, Jurkat, OVCAR-8, and SR cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-RBM22 antibody (orb1523927) used for WB at 0.04 µg/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.

Detection of human RBM22 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 5% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-RBM22 antibody (orb1523927) used for IP at 6 µg per reaction. RBM22 was also immunoprecipitated by a previous lot of this antibody (orb1523927) and a second antibody against a different epitope of RBM22.

Quick Database Links

Gene Symbol

RBM22

UniProt

Q9NW64

RefSeq (Protein):NP_060517.1

UniProt Details

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No UniProt data available

NCBI Reference Sequences

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Associated Accession Numbers

Curated reference sequences for the gene transcript and protein product

Protein	NP_060517.1

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Protocol Information

Western Blot (IB, immunoblot)

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Immunoprecipitation

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