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Rabbit anti-TFEB Antibody
SKU: orb1524402
Description
Images & Validation
−Item 1 of 3
| Tested Applications | IP, WB |
|---|---|
| Dilution range | WB - 1:2,000 - 1:10,000; IP - 2 - 10 µg/mg lysate |
| Reactivity | Human, Mouse |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Immunogen | Between 426 and C-terminus |
| Target | TFEB |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | 2 - 8°C |
|---|---|
| Form/Appearance | Whole IgG |
| Buffer/Preservatives | Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide |
| Concentration | 1000 µg/ml |
| Disclaimer | For research use only |
Alternative Names
−ALPHATFEB; transcription factor EB; TCFEB; BHLHE35; class E basic helix-loop-helix protein 35; T-cell transcription factor EB
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Detection of mouse TFEB by western blot. Samples: Whole cell lysate (10 µg) from NIH 3T3, CT26, CH27, TCMK-1, and BW5147.3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-TFEB antibody (orb1524402) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.
Detection of human TFEB by western blot. Samples: Whole cell lysate (10 µg) from HeLa, Hep-G2, MCF-7, Jurkat, and K-562 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-TFEB antibody (orb1524402) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Detection of human TFEB by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-TFEB antibody (orb1524402) used for IP at 6 µg per reaction. TFEB was also immunoprecipitated by a previous lot of this antibody (orb1524402) and a second antibody against a different epitope of TFEB.
Quick Database Links
UniProt Details
− No UniProt data available
NCBI Reference Sequences
−Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product| Protein | NP_009093.1 |
|---|
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Datasheet
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Protocol Information
WB
Western Blot (IB, immunoblot)
IP
Immunoprecipitation
Rabbit anti-TFEB Antibody (orb1524402)
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