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Rabbit anti-UVRAG Antibody
SKU: orb1527414
Description
Images & Validation
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| Tested Applications | IHC, IP, WB |
|---|---|
| Dilution range | WB - 1:2,000 - 1:10,000; IP - 2 - 5 µg/mg lysate; IHC - 1:100 - 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections. |
| Reactivity | Human |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Immunogen | Between 649 and C-terminus |
| Target | UVRAG |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | 2 - 8°C |
|---|---|
| Form/Appearance | Whole IgG |
| Buffer/Preservatives | Tris-buffered Saline containing 0.1% rAlbumin and 0.09% Sodium Azide |
| Concentration | 200 µg/ml |
| Disclaimer | For research use only |
Alternative Names
−p63; VPS38; UV radiation resistance-associated gene protein; DHTX; beclin 1 binding protein; disrupted in heterotaxy
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Detection of human UVRAG by western blot. Samples: Whole cell lysate from HeLa (5, 15, and 50 µg) and HEK293T (T; 50 µg) cells. Antibody: Affinity purified rabbit anti-UVRAG antibody orb1527414 used at 0.04 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
Detection of human UVRAG by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-UVRAG antibody orb1527414 used for IP at 3 µg/mg lysate.
Detection of human UVRAG by immunohistochemistry.Sample: FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti-UVRAG (Cat. No. orb1527414) used at a dilution of 1: 200 (1µg/ml). Detection: DAB
Quick Database Links
UniProt Details
− No UniProt data available
NCBI Reference Sequences
−Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product| Protein | NP_003360.2 |
|---|
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
IP
Immunoprecipitation
Rabbit anti-UVRAG Antibody (orb1527414)
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