Recombinant EGF (Epidermal growth factor), Human, Animal-Free

SKU: orb1179132

Description

Images & Validation

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Tested Applications	Cell Culture, ELISA

Key Properties

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Source	Escherichia coli
Expression System	Escherichia coli
Biological Origin	Human
Biological Activity	Measure by its ability to induce 3T3 cells proliferation. The ED50 for this effect is 0.05-0.12 ng/mL. The specific activity of recombinant human EGF is approximately >1.4 x 106 IU/mg.
Reactivity	Human
Tag	His-tag at the N-terminus
Protein Sequence	MNSDSECPLSHDGYCLHDGVCMYIEALDKYACNCVVGYIGERCQYRDLKWWELRLE with polyhistidine tag at the C-terminus.
Purity	>95% as determined by SDS-PAGE. Ni-NTA chromatography.
Endotoxins	<0.1 EU per 1 μg of the protein by the LAL method.

Storage & Handling

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Storage	Lyophilized protein should be stored at -20°C. Upon reconstitution, protein aliquots should be stored at -20°C or -80°C.
Form/Appearance	Lyophilized
Buffer/Preservatives	The protein was lyophilized from a solution containing 1X PBS, pH 8.0.
Reconstitution	It is recommended to reconstitute the lyophilized protein in sterile H2O to a concentration not less than 100 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.
Expiration Date	6 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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Urogastrone, URG

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NCBI Gene ID

1950

NCBI Gene Details

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No NCBI Gene data available

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Protocol Information

ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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