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Human ACE-2
SKU: orb2993542
Description
Images & Validation
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Key Properties
−| Expression System | Human Cells |
|---|---|
| Biological Origin | Human |
| Biological Activity | BLI: Loaded 2019-nCoV S Protein RBD-mFc(Cat#DRA32) on AMC Biosensor, can bind Human ACE-2-His(Cat#orb2993542) with an affinity constant of 2.06 nM as determined in BLI assay. |
| Tag | C-6His |
| Molecular Weight | Predicted: 84.63 KDa. Observed: 103 KDa, reducing conditions |
| Expression Region | Gln18-Ser740 |
| Purity | SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE. |
| Endotoxins | < 1 EU/µg as determined by LAL test. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Store at ≤-70°C, stable for 6 months after receipt. Store at ≤-70°C, stable for 3 months under sterile conditions after opening. Please minimize freeze-thaw cycles. |
|---|---|
| Form/Appearance | Supplied as a 0.2 μm filtered solution of 20mM Tris-HCl, 300mM NaCl, 1mM ZnCl2, 10% Glycerol, pH 7.4. |
| Disclaimer | For research use only |
Alternative Names
−Angiotensin-Converting Enzyme 2; ACE-Related Carboxypeptidase; Angiotensin-Converting Enzyme Homolog; ACEH; Metalloprotease MPROT15; ACE2
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The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
The protein has a predicted molecular mass of 109.7 kDa after removal of the signal peptide.
Mammalian
10 μg, 50 μg, 100 μg Rhesus Macaque ACE-2 [orb2993118]
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SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
Predicted: 110.6 KDa. Observed: 120-140 KDa, reducing conditions
10 μg, 50 μg, 500 μg, 1 mgHuman ACE-2 [orb2993136]
Unconjugated
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
Predicted: 110 KDa. Observed: 110-140 KDa, reducing conditions
1 mg, 50 μg, 500 μg, 10 μgBiotinylated Human ACE-2 [orb2993139]
Biotin
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
Predicted: 86.2 KDa. Observed: 110-140 KDa, reducing conditions
20 μg, 100 μgHuman ACE-2 [orb2993140]
Unconjugated
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.. SEC-HPLC: Greater than 95% as determined by SEC-HPLC. (QC verified)
Predicted: 110.5 KDa. Observed: 110-140 KDa, reducing conditions
50 μg, 500 μg, 1 mg, 10 μg
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Human ACE-2 (orb2993542)
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