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RFWD3 rabbit pAb
SKU: orb769586
Featured
Description
Images & Validation
−Item 1 of 4
| Tested Applications | ELISA, IHC-P, WB |
|---|---|
| Dilution range | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/40000. Not yet tested in other applications. |
| Reactivity | Human, Mouse, Rat |
Key Properties
−| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Immunogen | The antiserum was produced against synthesized peptide derived from human RFWD3. AA range:374-423 |
| Molecular Weight | 85 |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | PBS with 0.02% sodium azide and 50% glycerol pH 7.4. |
| Concentration | 1 mg/ml |
| Disclaimer | For research use only |
Alternative Names
−Anti-RFWD3 antibody, anti-RNF201 antibody, anti-E3 ubiquitin-protein ligase RFWD3 antibody, anti-RING finger and WD repeat domain-containing protein 3 antibody, anti-RING finger protein 201 antibody
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Western Blot analysis of 823 cells using RFWD3 Polyclonal Antibody diluted at 1:2000.
Western blot analysis of lysates from MCF-7 cells, using RFWD3 Antibody. The lane on the right is blocked with the synthesized peptide.
Western blot analysis of the lysates from HepG2 cells using RFWD3 antibody.
Immunohistochemical analysis of paraffin-embedded human Small intestinal stromal tumor. 1, Tris-EDTA, pH9.0 was used for antigen retrieval. 2 Antibody was diluted at 1:200 (4°C overnight.3, Secondary antibody was diluted at 1:200 (room temperature, 45min).
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC-P
Immunohistochemistry Paraffin
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
RFWD3 rabbit pAb (orb769586)
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