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RGS14 Antibody

SKU: orb18750

Description

Goat polyclonal to RGS14

Research Area

Epigenetics

Images & Validation

Tested ApplicationsELISA, FC, IF
Dilution RangeELISA: 1:16000, WB: 0.5-2 μg/ml
ReactivityHuman
Application Notes
ELISA: Peptide ELISA: antibody detection limit dilution 1:16000.WB: Approx 65-70kDa band observed in Jurkat lysates (predicted MW of 65kDa according to NP_006471). Recommended for use at 0.5-2 μg/ml.

Key Properties

HostGoat
ClonalityPolyclonal
TargetRGS14
Molecular Weight61.4
PurificationPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/PreservativesSupplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
DisclaimerFor research use only

Alternative Names

anti RGS14 antibody, anti regulator of G-protein signalling 14 antibody, anti regulation of G protein signaling 14 antibody

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RGS14 Antibody

Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml), showing nuclear and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml).

RGS14 Antibody

Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (1 ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

NCBI Gene Details

No NCBI Gene data available

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Protocol Information

FC
Flow Cytometry
View Protocol
IF
Immunofluorescence
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol

RGS14 Antibody (orb18750)

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Available Sizes

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100 μg
$ 530.00
DispatchUsually dispatched within 5-10 working days
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