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SARS-CoV-2 (COVID-19) Nucleocapsid Antibody [75G5a]
SKU: orb1274608
Description
Images & Validation
−Item 1 of 5
| Tested Applications | ELISA |
|---|---|
| Reactivity | Virus |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Recombinant |
| Isotype | IgG1k |
| Clone No. | 75G5a |
| Immunogen | SARS-CoV-2 nucleoprotein (NP) specific peptide |
| Target | N |
| Purification | SARS-CoV-2 (COVID-19) Nucleoprotein Antibody [75G5a] is affinity chromatography purified via peptide column. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | 1X PBS, 0.02% NaN3, 50% Glycerol, 0.1% rAlbumin |
| Concentration | batch dependent |
| Disclaimer | For research use only |
Alternative Names
−SARS-CoV-2 (COVID-19) Nucleoprotein Antibody [P001A]: Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)
Quality Guarantee
Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at support@biorbyt.com.
![SARS-CoV-2 (COVID-19) Nucleocapsid Antibody [75G5a]](/images/pub/media/catalog/product/NewWebsite/15/orb1274608_1.jpg)
Immunohistochemistry Validation of SARS-CoV-2 (COVID-19) Nucleocapsid in COVID-19 Patient Lung. Immunohistochemical analysis of paraffin-embedded COVID-19 patient lung tissue using anti-SARS-CoV-2 (COVID-19) Nucleocapsid antibody (orb1274608, 1 ng/mL). Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4 ˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin. Strong signal of SARS-COV-2 Nucleocapsid protein was observed in the macrophages of COVID-19 patient lung, but not in non-COVID-19 patient lung.
![SARS-CoV-2 (COVID-19) Nucleocapsid Antibody [75G5a]](/images/pub/media/catalog/product/NewWebsite/15/orb1274608_2.jpg)
Microtiter wells were coated with SARS-CoV-2 (COVID-19) Nucleocapsid Protein (NP) and SARS-CoV NP at 1 ug/mL. Purified rabbit monoclonal antibody was serially diluted 1:2 starting at 1 ug/mL, and shows very strong and specific binding to COVID-19 NP antigen, with no significant cross-reactivity to SARS-CoV NP antigen.
![SARS-CoV-2 (COVID-19) Nucleocapsid Antibody [75G5a]](/images/pub/media/catalog/product/NewWebsite/15/orb1274608_3.jpg)
A sandwich ELISA was performed using SARS-CoV-2 (COVID-19) Nucleocapsid Protein (NP) specific rabbit monoclonal antibodies. COVID-19 NP was serially diluted 1:2 starting at 20 ng/mL.
![SARS-CoV-2 (COVID-19) Nucleocapsid Antibody [75G5a]](/images/pub/media/catalog/product/NewWebsite/15/orb1274608_4.jpg)
A sandwich ELISA was performed using SARS-CoV-2 (COVID-19) Nucleocapsid Protein (NP) specific rabbit monoclonal antibodies. COVID-19 NP was serially diluted 1:2 starting at 25 ng/mL.
![SARS-CoV-2 (COVID-19) Nucleocapsid Antibody [75G5a]](/images/pub/media/catalog/product/NewWebsite/15/orb1274608_5.jpg)
Western blot analysis of SARS-CoV2 and SARS-CoV nucleocapsid protein (50 ng) probed with 1 ug/mL CoV2NP rabbit monoclonal Antibody.
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SARS-CoV-2 (COVID-19) Nucleocapsid Antibody [75G5a] (orb1274608)
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