SARS-CoV-2 (COVID-19) Spike RBD Neutralizing Antibody [C-A11]

SKU: orb1274601

Description

Images & Validation

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Item 1 of 4

Tested Applications	FC, NeA
Reactivity	Virus
Application Notes	Functional Assay: This antibody recognizes the SARS-CoV-2 Spike Protein RBD domain and inhibits the interaction between SARS-CoV-2 RBD and ACE2. Can be paired with other antibody for detection in sandwich ELISA format.

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Recombinant
Isotype	IgG1k
Clone No.	C-A11
Immunogen	recombinant protein (RBD)
Target	S
Purification	Protein A
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	1X PBS
Concentration	batch dependent
Disclaimer	For research use only

Alternative Names

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Spike protein S1, Spike protein S2, RBD domain, Binding to ACE-2 receptor, SARS-CoV-2 variants, U.K variant B.1.1.7, South Africa variant B.1.351

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SARS-CoV-2 (COVID-19) Spike RBD Neutralizing Antibody [C-A11]

Flow cytometry neutralizing assay: C-A11 displayed dose dependent blocking of spike proteins (wild type and UK variant) to ACE2 expressing cells.

Affinity assessment of SARS-CoV-2 C-A11 IgG to wild type and B.1.1.7 (UK) S1+S2 trimers. C-A11 possesses very low (pM) KD affinity to both variants.

Flow cytomery neutralizing assay of C-A11 (plotted graphs): ACE2 transfected cells were incubated with biotinylated spike protein (0.5 ug/mL) in the presence or absence of antibody. Binding of spike protein was detected with DyLight488 conjugated StreptAvidin. C-A11 efficiently blocked the binding of wild type and UK variant (B.1.1.7) to ACE2 expressing HEK293 cells.

Binding of C-A11 to SARS-CoV-2 variants (UK and South Africa): C-A11 shows good cross reactivity to both variants B.1.1.7 (UK) and B.1.351 (S. Africa).

Quick Database Links

Gene Symbol

UniProt

P0DTC2

RefSeq:P0DTC2

UniProt Details

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No UniProt data available

NCBI Reference Sequences

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Associated Accession Numbers

Curated reference sequences for the gene transcript and protein product

RefSeq	P0DTC2

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Protocol Information

Flow Cytometry

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