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SC-560

SKU: orb1303160
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Description

SC-560

Research Area

Immunology & Inflammation, Neuroscience

Images & Validation

Key Properties

CAS Number188817-13-2
MW352.74
Purity99.97%
FormulaC17H12ClF3N2O
SMILESCOc1ccc(cc1)-n1nc(cc1-c1ccc(Cl)cc1)C(F)(F)F
TargetCOX
Solubility10% DMSO+40% PEG300+5% Tween 80+45% Saline:2.5 mg/mL (7.09 mM);DMSO:35.3 mg/mL (100.07 mM)

Bioactivity

Target IC50
COX-2:6.3 μM|COX-1:9 nM.
In Vivo
Oral dosing with either 10 or 30 mg/kg SC-560 1 hour before assay completely inhibits ionophore-stimulated TxB2 production, indicating that SC-560 is orally bioavailable and inhibits COX-1 in vivo SC-560 extensively distributes into rat tissues, with a CL approaching hepatic plasma flow. however, after oral administration, it exhibits low < 5%), formulation-dependent bioavailability and demonstrates kidney toxicity.
In Vitro
Preincubation of cyclooxygenase-1 (COX-1) with SC-560 selectively inhibit the conversion of arachidonic acid to prostag andin E2 (PGE2) in a concentration dependent manner, demonstrating higher specificity for COX-1 over COX-2, as indicated by SC-560's inhibitory concentration 50 IC50 for COX-2 being 6.3 μM, nearly 1,000-fold greater than for COX-1. furthermore , SC-560 has been shown to suppress hepatocellular carcinoma (HCC) cell growth, colony formation in soft agar, and induce apoptosis in a dose- and time-dependent manner. Additionally, it downregulates anti-apoptotic proteins, including survivin and X-linked inhibitor of apoptosis protein (XIAP), while simultaneously activating caspases 3 and 7, illustrating its therapeutic potential in HCC treatment through multifaceted mechanisms of action.
Cell Research
SC-560 is dissolved in DMS. uH-6 and HA22T/VGH cells (5000/well) are treated with various concentration of SC-560 (5, 10, 25, 50, 100, 200 μM) and cultured for 72 h. A the end of treatment, cell viability is assessed by MTS assay.
Animal Research
Rat the pharmacokinetics of SC-560 is studied in Sprague Dawley rats after a Single intravenous (i.v.) and oral dose (10 mg/kg) in polyethylene glycol (PEG) 600 and a Single oral dose (10 mg/kg) in 1% methylcellulose (MC). Serial blood Samples are collected via a catheter inserted in the right jugular vein and serum Samples are analysed for SC-560 using reverse phase HPLC. After oral administration of SC-560 in PEG, urine is also collected for 24 h and analyzed for urinary sodium, chloride, and potassium as well as NAG.

Storage & Handling

StoragePowder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
DisclaimerFor research use only

Alternative Names

Cyclooxygenase, COX-2, COX-1, COX, inhibit, Inhibitor, SC 560, SC560, SC-560

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    188817-13-2

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    C17H12ClF3N2O

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Key Properties

No computed properties available.

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Protocol Information

SC-560 (orb1303160)

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% DMSO +
%+
% Tween 80 +
%

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5 mg
$ 170.00
25 mg
$ 340.00
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