SERCA1 rabbit pAb Antibody

SKU: orb766303

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Description

Rabbit polyclonal antibody to SERCA1

Images & Validation

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Item 1 of 3

Tested Applications	ELISA, IF, IHC, WB
Dilution Range	Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/10000. Not yet tested in other applications.
Reactivity	Amphibian, Human, Mouse, Porcine, Rat

Key Properties

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Clonality	Polyclonal
Isotype	IgG
Immunogen	The antiserum was produced against synthesized peptide derived from human ATP2A1. AA range:548-597
Molecular Weight	100
Purification	The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
Concentration	1 mg/ml
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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Anti-ATP2A1 antibody, anti-Sarcoplasmic/endoplasmic reticulum calcium ATPase 1 antibody, anti-SERCA1 antibody, anti-SR Ca(2+)-ATPase 1 antibody, anti-Calcium pump 1 antibody, anti-Calcium-transporting ATPase sarcoplasmic reticulum type antibody, anti- fast twitch skeletal muscle isoform antibody, anti- antibody

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Western Blot analysis of various cells using SERCA1 Polyclonal Antibody diluted at 1:2000.

Western blot analysis of lysates from HT-29, A549, and NIH/3T3 cells, using ATP2A1 Antibody. The lane on the right is blocked with the synthesized peptide.

Immunohistochemical analysis of paraffin-embedded human Breast cancer. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).

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Protocol Information

Western Blot (IB, immunoblot)

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IHC

Immunohistochemistry

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Immunofluorescence

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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