SERPINF1/PEDF Antibody

SKU: orb1248124

Description

Research Area

Cancer Biology, Neuroscience, Signal Transduction

Images & Validation

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Item 1 of 1

Tested Applications	ELISA, WB
Reactivity	Human
Predicted Reactivity	Bovine, Canine, Porcine
Application Notes	Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 48kDa band observed in Human Albumin-depleted Serum and Plasma lysates (calculated MW of 46.3kDa according to NP_002606.3). Recommended concentration: 0.3-1µg/mL.

Key Properties

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Antibody Type	Primary Antibody
Host	Goat
Clonality	Polyclonal
Immunogen	The immunogen for this antibody is: C-KITGKPIKLTQVEHR
Target	SERPINF1 / PEDF
Molecular Weight	Approx 48kDa
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration	500 ug/mL
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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SERPINF1, EPC-1, PEDF, PIG35, serpin peptidase inhibitor, clade F (alpha-2 antiplasmin, pigment epithelium derived factor), member 1, pigment epithelium-derived factor, proliferation-inducing protein 35, serine (or cysteine) proteinase inhibitor, clade F (alpha-2 antiplasmin, pigment epithelium derived factor), member 1

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orb1248124 (0.3 ug/ml) staining of Human Albumin-depleted Plasma (A) and Serum (B) lysates (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Quick Database Links

Gene Symbol

SERPINF1 / PEDF

UniProt

P36955

RefSeq (Protein):NP_002606.3

UniProt Details

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No UniProt data available

NCBI Reference Sequences

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Associated Accession Numbers

Curated reference sequences for the gene transcript and protein product

Protein	NP_002606.3

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Protocol Information

Western Blot (IB, immunoblot)

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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