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SFRP2 Antibody
SKU: orb535892
Description
Research Area
Cardiovascular Research, Signal Transduction, Stem Cell & Developmental Biology
Images & Validation
−Item 1 of 2
| Tested Applications | IHC, WB |
|---|---|
| Dilution range | WB (1:1000); IHC (1:50) |
| Reactivity | Human, Mouse |
| Application Notes |
Key Properties
−| Host | Rabbit |
|---|---|
| Clonality | Polyclonal |
| Immunogen | Synthetic peptide from the C-terminal of Human Secreted frizzled-related protein 2 (aa. 195-295) |
| Target | SFRP2 |
| Molecular Weight | 37 kDa |
| Purification | Peptide Affinity Purified |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | PBS pH 7.4, 50% glycerol, 0.09% sodium azide *Storage buffer changes when conjugated |
| Concentration | 1 mg/ml |
| Disclaimer | For research use only |
Alternative Names
−SDF5, Secreted frizzled-related protein 2, FRP 2, UNQ361/PRO697, sFRP-2, FKSG12, SFRP2_HUMAN, MGC153618, Frizzled related protein 2, MGC53344, FRP2, FRP-2, SARP1, AI851596, SARP 1, MGC128911, SARP-1, Secreted apoptosis related protein 1, ORF Names:, Secreted apoptosis-related protein 1, SDF 5, Sfrp2 protein, sFRP 2, Secreted frizzled related protein 2
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Immunohistochemistry analysis using Rabbit Anti-SFRP2 Polyclonal Antibody. Tissue: Liver. Species: Human. Fixation: Formalin Fixed Paraffin-Embedded. Primary Antibody: Rabbit Anti-SFRP2 Polyclonal Antibody at 1:50 for 30 min at RT. Counterstain: Hematoxylin. Magnification: 20X. HRP-DAB Detection.
Western blot analysis of Mouse Brain showing detection of 33.5 kDa SFRP2 protein using Rabbit Anti-SFRP2 Polyclonal Antibody. Load: 15 μg. Block: 5% Skim Milk powder in TBST. Primary Antibody: Rabbit Anti-SFRP2 Polyclonal Antibody at 1:1000 for 2 hours at RT with shaking. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:5000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: 33.5 kDa. Other Band (s): 37 kDa.
Quick Database Links
UniProt Details
− No UniProt data available
NCBI Gene Details
− No NCBI Gene data available
NCBI Reference Sequences
−Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product| Protein | NP_003004.1 |
|---|
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Datasheet
Product Information
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
SFRP2 Antibody (orb535892)
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