TIMM17A Antibody

SKU: orb1260346

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Description

TIMM17A Antibody

Research Area

Cancer Biology, Signal Transduction

Images & Validation

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Item 1 of 3

Tested Applications	IF, IHC, WB
Reactivity	Human, Mouse
Application Notes	WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 1-171 of human TIMM17A (NP_006326.1).
Target	TIMM17A
Molecular Weight	Observed: 18kDa
Purification	Affinity purification
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
Concentration	batch dependent
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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TIM17, TIM17A, mitochondrial import inner membrane translocase subunit Tim17-A, inner membrane preprotein translocase Tim17a, mitochondrial inner membrane translocase, preprotein translocase, translocase of inner mitochondrial membrane 17 homolog A

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Western blot analysis of extracts of various cell lines, using TIMM17A antibody (orb1260346) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Immunohistochemistry of paraffin-embedded human liver cancer using TIMM17A antibody (orb1260346) at dilution of 1:100 (40x lens).

Immunofluorescence analysis of U2OS cells using TIMM17A antibody (orb1260346). Blue: DAPI for nuclear staining.

Quick Database Links

Gene Symbol

TIMM17A

UniProt

Q99595

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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IHC

Immunohistochemistry

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Immunofluorescence

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