TLR4/MD-2, Mouse, mAb MTS510 Antibody

SKU: orb1109178

Description

Mouse monoclonal antibody to TLR4/MD-2

Images & Validation

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Tested Applications	FC, Functional Studies, IF, IHC-Fr, IP
Dilution range	For immunohistology and flow cytometry dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50.
Reactivity	Mouse
Application Notes	F: 6 µm acetone fixed sections blocked with 0.3% hydrogen peroxidase in methanol and subsequently with normal serum. Sections were incubated for 2h at RT at 1/100 dilution of mAb Fc: Cells were incubated with 0.1um mAb for 30 minutes at 4°C FS: Cells were pre-incubated with 10 µg/ml of antagonistic mAb MTS510 RAW264.7 cells (105) were stained with 4µg/ml mAb for 1h at 4°C. Read more...

Key Properties

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Clonality	Monoclonal
Isotype	Rat IgG2a
Immunogen	Ba/F3 cells expressing mouse TLR4 and MD-22
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	1 ml (100 µg/ml) 0.2 µm filtered antibody solution in PBS and 0.1% bovine serum albumin.
Buffer/Preservatives	Quantities >500 µg will be sent without preservatives and are carrier-free.
Disclaimer	For research use only

Alternative Names

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Toll-like receptor 4:- TLR4, CD284, ARMD10 MD2: ESOP-1; myeloid differentiation protein-2; lymphocyte antigen 96 (Ly-96)

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Protocol Information

IHC-Fr

Immunohistochemistry Frozen

Flow Cytometry

Immunofluorescence

Immunoprecipitation