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Troponin I-C (phospho Ser43) rabbit pAb Antibody
SKU: orb770281
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Description
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| Tested Applications | ELISA, IF, IHC, WB |
|---|---|
| Dilution range | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/40000. Not yet tested in other applications. |
| Reactivity | Human, Mouse, Rat |
Key Properties
−| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Immunogen | The antiserum was produced against synthesized peptide derived from human TNNI3 around the phosphorylation site of Ser43. AA range:11-60 |
| Molecular Weight | 26 |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | PBS with 0.02% sodium azide and 50% glycerol pH 7.4. |
| Concentration | 1 mg/ml |
| Disclaimer | For research use only |
Alternative Names
−Anti-TNNI3 antibody, anti-TNNC1 antibody, anti-Troponin I antibody, anti- cardiac muscle antibody, anti-Cardiac troponin I antibody
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Western blot analysis of lysates from Jurkat cells, using TNNI3 (Phospho-Ser43) Antibody. The lane on the right is blocked with the phospho peptide.
Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using TNNI3 (Phospho-Ser43) Antibody.
Immunohistochemistry analysis of paraffin-embedded human heart, using TNNI3 (Phospho-Ser43) Antibody. The picture on the right is blocked with the phospho peptide.
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
IF
Immunofluorescence
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
Troponin I-C (phospho Ser43) rabbit pAb Antibody (orb770281)
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