Urocortin-2, Human, mAb 2D7 Antibody

SKU: orb1108821

Description

Mouse monoclonal antibody to Urocortin-2

Images & Validation

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Tested Applications	IA, WB
Dilution range	Dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50.
Reactivity	Human
Application Notes	IA: Antibody 2D7 can be used as capture antibody (Ref.1). W: Protein samples were separated by 16% SDS-PAGE for an hour at 200 V and electroblotted onto Immun-Blot PVDF membrane at 100 mA for 1 h. Western blotting was performed using mouse 2D7 (1/1000 dilution) as primary antibody and goat anti mouse IgG conjugated to horse radish peroxidase (1/2000). The band sizes are 35 and 80 kDa. (Ref.1)

Key Properties

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Clonality	Monoclonal
Isotype	Mouse IgG1
Immunogen	IMAC-purified SUMO-eXact-preproUCN2 (Ref.1)
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	1 ml (100 µg/ml) 0.2 µm filtered protein G purified antibody solution in PBS, 0.1% bovine serum albumin and 0.02% sodium azide.
Buffer/Preservatives	0.2 ml (100 µg/ml) 0.2 µm filtered protein G purified antibody solution in PBS, 0.1% bovine serum albumin and 0.02% sodium azide.
Disclaimer	For research use only

Alternative Names

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Stresscopin-related peptide, Urocortin II, Ucn II, Urocortin-related peptide Gene name: UCN2, SRP, URP

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Quick Database Links

UniProt

Q96RP3

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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