2-Fucosyllactose

SKU: orb1940886

Description

2'-Fucosyllactose (2'-FL) is an oligosaccharide that could be derived from human milk. 2'-Fucosyllactose regulates the expression of CD14, alleviates colitis and regulates the gut microbiome. 2'-Fucosyllactose stimulates T cells to increase IFN-γ production and decreases IL-6, IL-17, and TNF-α production of cytokines.

Images & Validation

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Key Properties

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CAS Number	41263-94-9
MW	488.44
Purity	>98% (HPLC)
Formula	C₁₈H₃₂O₁₅
SMILES	O=C[C@H](O)[C@@H](O)[C@@H]([C@H](O)CO)O[C@H]1[C@@H]([C@H]([C@H]([C@H](O1)CO)O)O)O[C@H]2[C@H]([C@@H]([C@@H]([C@@H](O2)C)O)O)O
Target	TNF
Solubility	In Vitro: H2O : 50 mg/mL (102.37 mM)

Bioactivity

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In Vivo

2'-Fucosyllactose (2'-FL; 100 mg (200 μL); i. g. ; daily, for 4 d; C57BL/6 mice with AIEC infection) inhibits AIEC infection and inflammation In vivo. Animal model: C57BL/6 mice (8 weeks) with AIEC (uropathogenic E. coli, and adherent-invasive E. coli) infection. Dosage: 100 mg (200 μL). Administration: Oral gavage; daily, for 4 days. Result: Had colons lengths were closer to normal. Inhibited the colonisation of the colonic mucosa by O83-positive bacteria. Decreased CD14 expression, CD14 mRNA levels, IL-6, IL-17 and TNF-α levels in colonic.

In Vitro

2'-Fucosyllactose (2'-FL; 0-12 mg/mL; 48 h) suppress cell-associated CD14 expression and to attenuate LPS (100 μg/mL) stimulated IL-8 secretion in T84 cells.2'-Fucosyllactose (2 mg/mL; 48 h; T84 and HCT8 cells) ameliorates inflammation induced by bacterial invasion. 2'-Fucosyllactose inhibits ETEC invasion and attenuated the consequent IL-8 secretion.2'-Fucosyllactose (2 mg/mL; 48 h; T84 and HCT8 cells) induces macrophage migration inhibitory factor signal pathways that suppress inflammation. Western blot analysis. Cell line: T84 cells. Concentration: 0, 2, and 4 mg/mL. Incubation time: 48 hours. Result: Suppressed CD14 mRNA and reduced cell-associated CD14 protein expression. Western blot analysis. Cell line: T84 and HCT8 cells T84 and HCT8 cells. Concentration: 2 mg/mL. Incubation time: 48 hours. Result: Suppressed CD14 mRNA and reduced cell-associated CD14 protein expression. Inhibited CD14 and NF-κB induction. Induced iκB and SOCS2 expression and STAT3 phosphorylation.

Storage & Handling

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Storage	Storage temperature: -20°C. Stability: ≥ 2 years
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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2'-FL

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Gene Symbol

TNF

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