4-Methylumbelliferyl oleate

SKU: orb1944592

Description

4-Methylumbelliferyl oleate is a fluorogenic substrate for lipase activity assays, with excitation/emission at 320/450 nm. Its cleavage enables the quantification of lipase activity and is widely used in vitro for screening pancreatic lipase inhibitors in biochemical and pharmaceutical research.

Images & Validation

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Key Properties

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CAS Number	18323-58-5
MW	440.62
Purity	99.64% (May vary between batches)
Formula	C₂₈H₄₀O₄
SMILES	CCCCCCCC\C=C/CCCCCCCC(=O)Oc1ccc2c(C)cc(=O)oc2c1
Target	Others
Solubility	DMSO:80 mg/mL (181.56 mM);10% DMSO+40% PEG300+5% Tween-80+45% Saline:3.3 mg/mL (7.49 mM)

Bioactivity

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In Vitro

Pancreatic lipase inhibitory activity was assessed by employing 4-Methylumbelliferyl (4-MU) oleate as the substrate. The assay mixture contained 0.1 mM 4-MU oleate (50 µL), McIlvaine buffer (20 µL of 0.1 M citrate-Na2HPO4, pH 7.4), and the test sample (5 µL). This was followed by the addition of porcine pancreatic lipase (25 µL), adjusting the total volume to 0.1 mL. Post incubation at 37℃ for 10 minutes, lipase-catalyzed liberation of 4-MU was quantified using fluorescence multi-detection reader at excitation and emission wavelengths of 320 nm and 450 nm, respectively .

Storage & Handling

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Storage	keep away from moisture \| store at -20°C \| Shipping with blue ice/Shipping at ambient temperature.
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

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Compound Identifiers

PubChem CID

6436487 ↗

Key Properties

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No computed properties available.

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