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ACACA Antibody
SKU: orb1259430
Featured
Description
Images & Validation
−Item 1 of 4
| Tested Applications | IF, IHC, WB |
|---|---|
| Reactivity | Human, Mouse, Rat |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | A synthetic peptide of human ACACA |
| Target | ACACA |
| Molecular Weight | Observed: 240kDa |
| Purification | Affinity purification |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | PBS with 0.02% sodium azide, 50% glycerol, pH 7.3. |
| Concentration | batch dependent |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−Acetyl-CoA carboxylase 1, ACC1, ACC-alpha, Biotin carboxylase, ACACA, ACAC, ACC1, ACCA
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Western blot analysis of extracts of various cell lines, using ACACA antibody (orb1259430) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
Immunofluorescence analysis of 293T cells using ACACA antibody (orb1259430) at dilution of 1:100. 293T cells were treated by Hydrogen Peroxide (2 nM) at 37 ℃ for 15 minutes after serum-starvation overnight (left). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of C6 cells using ACACA antibody (orb1259430) at dilution of 1:100. C6 cells were treated by Hydrogen Peroxide (2 nM) at 37 ℃ for 15 minutes after serum-starvation overnight (left). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of HeLa cells using ACACA antibody (orb1259430) at dilution of 1:100. HeLa cells were treated by Hydrogen Peroxide (2 nM) at 37 ℃ for 15 minutes after serum-starvation overnight (left). Blue: DAPI for nuclear staining.
Quick Database Links
Gene Symbol
ACACA
UniProt
UniProt Details
− No UniProt data available
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Datasheet
Product Information
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
IF
Immunofluorescence
ACACA Antibody (orb1259430)
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