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ACE2 protein

SKU: orb705121
ActiveBiologically Active

Description

This ACE2 protein spans the amino acid sequence from region 18-740aa. Purity: Greater than 94.8% as determined by SDS-PAGE.

Research Area

Epigenetics

Images & Validation

Application Notes
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Key Properties

SourceMammalian cell
Biological OriginPaguma larvata (Masked palm civet)
Biological Activity①Measured by its binding ability in a functional ELISA. Immobilized SARS-CoV S-RBD at 2 μg/ml can bind Paguma larvata ACE2, the EC50 is 5.056-7.559 ng/ml.②Recombinant Paguma larvata ACE2 protein enzyme activity is measured by its ability to cleave fluorogenic peptide substrate(Mca-Ala-Pro-Lys(Dnp)-OH), The Km is 22.84 μM.
TagC-terminal hFc1-tagged
Expression Region18-740aa
Protein LengthPartial
EndotoxinsLess than 1.0 EU/μg as determined by LAL method.
MW112.7 kDa
PurityGreater than 94.8% as determined by SDS-PAGE.
Protein SequenceQSTTEELAKTFLETFNYEAQELSYQSSVASWNYNTNITDENAKNMNEAGAKWSAYYEEQSKLAQTYPLAEIQDAKIKRQLQALQQSGSSVLSADKSQRLNTILNAMSTIYSTGKACNPNNPQECLLLEPGLDNIMENSKDYNERLWAWEGWRAEVGKQLRPLYEEYVALKNEMARANNYEDYGDYWRGDYEEEWTGGYNYSRNQLIQDVEDTFEQIKPLYQHLHAYVRAKLMDTYPSRISRTGCLPAHLLGDMWGRFWTNLYPLTVPFGQKPNIDVTDAMVNQNWDARRIFKEAEKFFVSVGLPNMTQGFWENSMLTEPGDGRKVVCHPTAWDLGKGDFRIKMCTKVTMDDFLTAHHEMGHIQYDMAYAAQPFLLRNGANEGFHEAVGEIMSLSAATPNHLKTIGLLSPAFSEDNETEINFLLKQALTIVGTLPFTYMLEKWRWMVFKGAIPKEQWMQKWWEMKRNIVGVVEPVPHDETYCDPASLFHVANDYSFIRYYTRTIYQFQFQEALCQIAKHEGPLHKCDISNSTEAGKKLLEMLSLGRSEPWTLALERVVGAKNMNVTPLLNYFEPLFTWLKEQNRNSFVGWDTDWRPYSDQSIKVRISLKSALGEKAYEWNDNEMYLFRSSIAYAMREYFSKVKNQTIPFVEDNVWVSDLKPRISFNFFVTFSNNVSDVIPRSEVEDAIRMSRSRINDAFRLDDNSLEFLGIEPTLSPPYRPPVT

Storage & Handling

StorageThe shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
Form/AppearanceLyophilized powder
Buffer/PreservativesLyophilized from a 0.2 μm sterile filtered PBS, 6% Trehalose, pH 7.4
Expiration Date6 months from date of receipt.
DisclaimerFor research use only

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ACE2 protein

(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

ACE2 protein

Measured by its binding ability in a functional ELISA. Immobilized SARS-CoV S-RBD at 2 μg/ml can bind Paguma larvata ACE2, the EC50 is 5.056-7.559 ng/ml.

ACE2 protein

Recombinant Paguma larvata ACE2 protein enzyme activity is Measured by its ability to cleave fluorogenic peptide substrate (Mca-Ala-Pro-Lys (Dnp) -OH), The Km is 22.84 μM.

ACE2 protein

The purity of ACE2 was greater than 90% as determined by SEC-HPLC

UniProt Details

No UniProt data available

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ACE2 protein (orb705121)

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20 μg
$ 210.00
100 μg
$ 390.00
1 mg
$ 2,820.00
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