Acetaminophen

SKU: orb1310976

Description

Acetaminophen

Research Area

Epigenetics & Chromatin, Immunology & Inflammation, Metabolism Research, Neuroscience

Images & Validation

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Key Properties

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CAS Number	103-90-2
MW	151.16
Purity	99.92%
Formula	C₈H₉NO₂
SMILES	O=C(NC1=CC=C(O)C=C1)C
Target	Endogenous Metabolite,Histone Acetyltransferase,COX
Solubility	Ethanol:15.1 mg/mL (99.89 mM);H2O:20 mg/mL (132.31 mM);DMSO:237 mg/mL (1567.88 mM)

Bioactivity

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Target IC50	HepG2 cells:108.6 µg/ml\|COX-2:25.8 μM\|COX-1:113.7 μM\|MCF-7 cells:108.4 µg/ml\|COX-3:64 μM
In Vivo	METHODS: To detect hepatotoxicity in vivo, Acetaminophen was administered intraperitoneally to mice (300 mg/kg) and rats (1 g/kg). RESULTS: Extensive liver necrosis was observed in mice, but little damage was observed in rat samples. The rats were highly resistant to Acetaminophen-induced liver injury. METHODS: To test for in vivo hepatotoxicity, Acetaminophen was administered to aged and weakened mice acutely (300 mg/kg by gavage), chronically (100 mg/kg by diet once daily for six weeks), or subacutely (250 mg/kg by gavage three times daily for three days). RESULTS: There was no overall increase in Acetaminophen hepatotoxicity with age or frailty in mice, despite changes in certain pathways that would be expected to affect susceptibility to Acetaminophen toxicity. Read more...
In Vitro	METHODS: Non-melanoma and melanoma cell lines were treated with Acetaminophen (100 µM) for 48 h and cell viability was measured using MTT Assay. RESULTS: Acetaminophen showed considerable toxicity in melanoma cell lines SK-MEL-5, MeWo, B16-F0 and B16-F10, resulting in 40±3%, 45±7%, 66±8% and 60±5% cytotoxicity, respectively. Acetaminophen showed negligible toxicity at 100 μM in the non-melanoma cell lines PC-3, BJ, Saos-2 and SW-620 cells. METHODS: Neuroblastoma cells SH-SY5Y were treated with Acetaminophen (2 mM) for 24-48 h, and the expression levels of target proteins were detected using Western Blot. RESULTS: Acetaminophen induced cytochrome c release from mitochondria in a time-dependent manner, reaching a maximum level after 48 h of treatment. In addition, immunoblot analysis of cytoplasmic and mitochondrial fractions showed that Acetaminophen was able to induce the accumulation of Bax into mitochondria at 24 h after treatment. Read more...
Cell Research	Cells are exposed to Acetaminophen for 48 hours. Cell viability is determined by the trypan blue exclusion method. Intracellular GSH is measured by recording the disulfide, GS-TNB and 5-thio-nitrobenzoic acid (TNB), the yellow colored compound formed by the reaction between GSH with DTNB.(Only for Reference) Read more...

Storage & Handling

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Storage	The compound is unstable in solution. Please use soon \| Powder: -20°C for 3 years \| Shipping with blue ice/Shipping at ambient temperature.
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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Cyclooxygenase, COX-2, COX, COX-1, Acetaminophen, 4-Acetamidophenol, 4'-Hydroxyacetanilide, APAP, inhibit, Paracetamol, Inhibitor, HATs, HAT, Histone Acetyltransferase, HistoneAcetyltransferase, EndogenousMetabolite, Endogenous Metabolite

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Compound Identifiers

PubChem CID

1983 ↗

Key Properties

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No computed properties available.

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Acetaminophen (orb1310976)

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Applications:	IA, IHC-P
Reactivity:	Chemical
Species/Host:	Sheep
Clonality:	Polyclonal
Conjugation:	Unconjugated

Purity:	>98%
CAS Number:	1510-21-0
MW:	486.726310253143
Formula:	C₃₁H₅₀O₄

Purity:	>97%
CAS Number:	2377770-85-7
MW:	639.617
Formula:	C₂₇H₂₄F₃N₃O₈S₂

Purity:	>98%
CAS Number:	50700-49-7
MW:	149.146682024002
Formula:	C₈H₇NO₂

CAS Number:	2570985-89-4
MW:	488.585
Formula:	C₂₆H₃₂N₈O₂

Acetaminophen

Description

Research Area

Images & Validation

Key Properties

Bioactivity

Storage & Handling

Alternative Names

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