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H2B K15ac Antibody
SKU: orb1259420
Description
Images & Validation
−Item 1 of 8
| Tested Applications | IF, IHC, IP, WB |
|---|---|
| Reactivity | Human, Mouse |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | AA synthetic acetylated peptide corresponding to residues surrounding K15 of human Histone H2B |
| Target | H2B K15ac |
| Purification | Affinity purification |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | PBS with 0.02% sodium azide, 50% glycerol, pH 7.3. |
| Concentration | batch dependent |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Quality Guarantee
Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at support@biorbyt.com.
Western blot analysis of extracts of various cell lines, using Acetyl-Histone H2B-K15 antibody (orb1259420) at 1:1000 dilution. HeLa cells were treated by TSA (1 uM) at 37 ℃ for 18 hours. NIH/3T3 cells were treated by TSA (1 uM) at 37 ℃ for 18 hours. C6 cells were treated by TSA (1 uM) at 37 ℃ for 18 hours. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 10s.
Immunohistochemistry of paraffin-embedded rat lung using H2B K15ac antibody (orb1259420) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human breast cancer using H2B K15ac antibody (orb1259420) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human gastric cancer using H2B K15ac antibody (orb1259420) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using H2B K15ac antibody (orb1259420) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of C6 cells using Acetyl-Histone H2B-K15 antibody (orb1259420) at dilution of 1:100. C6 cells were treated by TSA (1 uM) at 37 ℃ for 18 hours. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of HeLa cells using Acetyl-Histone H2B-K15 antibody (orb1259420) at dilution of 1:100. HeLa cells were treated by TSA (1 uM) at 37 ℃ for 18 hours. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of NIH/3T3 cells using Acetyl-Histone H2B-K15 antibody (orb1259420) at dilution of 1:100. NIH/3T3 cells were treated by TSA (1 uM) at 37 ℃ for 18 hours. Blue: DAPI for nuclear staining.
Quick Database Links
Gene Symbol
H2B K15ac
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Datasheet
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
IF
Immunofluorescence
IP
Immunoprecipitation
H2B K15ac Antibody (orb1259420)
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