ACHE Antibody (N-term)

SKU: orb38623

Featured

Description

Rabbit polyclonal antibody to ACHE which is also known as acetylcholinesterase whose expression can be detected in the blood cells but it is majorly expressive in the erythrocytes. It is found in the post synapse. It performs several functions like protein self-association and serine hydrolase activity. It can terminate signal transduction at the neuromuscular junction by rapid hydrolysis of the acetylcholine released into the synaptic cleft and has a role in neuronal apoptosis.

Research Area

Signal Transduction

Images & Validation

−

Item 1 of 4

Tested Applications	FC, IF, IHC-P, WB
Dilution Range	WB: 1:1000, IHC-P: 1:10-50, FACS: 1:10-50, IF/ICC: 1:10-50
Reactivity	Human
Predicted Reactivity	Guinea pig

Key Properties

−

Host	Rabbit
Clonality	Polyclonal
Isotype	Rabbit IgG
Clone No.	BTD38914
Immunogen	Synthetic Peptide
Target	ACHE
Molecular Weight	67796
Conjugation	Unconjugated

Storage & Handling

−

Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Form/Appearance	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

−

Anti-Acetylcholinesterase antibody, anti-AChE antibody, anti-ACHE antibody

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at support@biorbyt.com.

ACHE Antibody (N-term) western blot analysis in Jurkat, Raji, Y79 cell line lysates (35 ug/lane). This demonstrates the ACHE antibody detected the ACHE protein (arrow).

ACHE Antibody (N-term) flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Confocal immunofluorescent analysis of ACHE Antibody (N-term) with NCI-H460 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).

Formalin-fixed and paraffin-embedded human brain tissue reacted with ACHE antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.