ADA Antibody (HRP)

Biorbyt biotin conjugated anti adenosine deaminase was used to detect adenosine deaminase in mouse pancreas lysate (Left, Lane 1, 30 ul) under reducing conditions. The antibody was also used to detect purified Adenosine Deaminase (right, Lane 2), and endogenous Adenosine Deaminase in whole cell lysate from Jurkat and Raji cells (1:1 mixture, lane 3) as well as Mouse Pancreas and Liver (1:1 mixture, lane 4). Lysates were run on 4-20% gel 140V under reducing conditions, transferred for 30 minutes at 100 V and blocked with 3% Fish Gel (left) or 3% BSA. Blot was incubated (1:5K in TBS, ON 4°C), washed 3X in TBS and incubated for 30 minutes with Dylight 488 conjugated Streptavidin (1:5K in orb348637).

Western Blot of Rabbit anti-Adenosine Deaminase Antibody Peroxidase Conjugated. Lane 1: Adenosine Deaminase (Calf Spleen). Load: 50 ng per lane. Primary antibody: Rabbit anti-Adenosine Deaminase Antibody Peroxidase Conjugated at 1:1000 overnight at 4°C. Secondary antibody: n/a. Block: orb348637 for 30 min at RT. Predicted/Observed size: 41 kDa, 45 kDa for Adenosine Deaminase (Calf Spleen).