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AHA1 Antibody
Description
Images & Validation
−| Tested Applications | ELISA, ICC, IF, IHC, IP, WB |
|---|---|
| Dilution range | WB (1:1000), ICC/IF (1:60); optimal dilutions for assays should be determined by the user. |
| Reactivity | Human, Mouse, Rat |
| Application Notes |
Key Properties
−| Host | Rabbit |
|---|---|
| Clonality | Polyclonal |
| Immunogen | Recombinant Full Length Mouse Aha1 Protein |
| Target | AHA1 |
| Molecular Weight | 38kDa |
| Purification | Protein A Purified |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | PBS pH 7.4, 50% glycerol, 0.09% sodium azide *Storage buffer changes when conjugated |
| Concentration | 1 mg/ml |
| Disclaimer | For research use only |
Alternative Names
−Similar Products
−AHA1/AHSA1 Antibody [orb1145840]
ELISA, FC, ICC, IF, IHC, WB
Human, Mouse, Rat
Rabbit
Polyclonal
Unconjugated
100 μgAHA-1 rabbit pAb Antibody [orb764487]
ELISA, IF, IHC, WB
Human, Mouse, Rat
Polyclonal
Unconjugated
100 μl, 50 μl

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Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-AHA1 Polyclonal Antibody. Tissue: Heat Shocked Cervical cancer cell line (HeLa). Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-AHA1 Polyclonal Antibody at 1:60 for 12 hours at 4°C. Secondary Antibody: R-PE Goat Anti-Rabbit (yellow) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Cytoplasm. Endoplasmic reticulum. Magnification: 100x. (A) DAPI (blue) nuclear stain. (B) Anti-AHA1 Antibody. (C) Composite. Heat Shocked at 42°C for 1h.

Western blot analysis of multiple Cell line lysates showing detection of AHA1 protein using Rabbit Anti-AHA1 Polyclonal Antibody. Load: 15 μgprotein. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Rabbit Anti-AHA1 Polyclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Donkey Anti-Rabbit IgG: HRP for 1 hour at RT.
Quick Database Links
UniProt Details
−NCBI Gene Details
−NCBI Reference Sequences
−| Protein | NP_666148.1 |
|---|
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Protocol Information
AHA1 Antibody (orb1822419)
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