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AIFM1 Antibody
SKU: orb1248086
Description
Research Area
Cell Biology
Images & Validation
−Item 1 of 6
| Tested Applications | ELISA, IF, WB |
|---|---|
| Reactivity | Human, Mouse, Porcine, Rat |
| Predicted Reactivity | Bovine, Canine |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Goat |
| Clonality | Polyclonal |
| Immunogen | The immunogen for this antibody is: C-NEVAKLFNIHED |
| Target | AIFM1 |
| Molecular Weight | Approx 70kDa |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
| Concentration | 500 ug/mL |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−AIFM1, apoptosis-inducing factor, mitochondrion-associated, 1, AIF, CMTX4, COWCK, COXPD6, PDCD8, apoptosis-inducing factor 1, mitochondrial, programmed cell death 8 (apoptosis-inducing factor), striatal apoptosis-inducing factor
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orb1248086 (0.01 ug/ml) staining of Jurkat lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.
orb1248086 (0.1 ug/ml) staining of NIH3T3 lysate (35 ug protein in RIPA buffer) Detected by chemiluminescence.
orb1248086 (0.01 ug/ml) staining of Mouse (A), Rat (B) and Pig (C) Heart lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.
orb1248086 (0.03 ug/ml) staining of Mouse (A) and Rat (B) Kidney lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence
orb1248086 Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (4 ug/ml), showing Mitochondrial staining.
orb1248086 Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (4 ug/ml), showing Mitochondrial staining.
Quick Database Links
UniProt Details
− No UniProt data available
NCBI Reference Sequences
−Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product| Protein | NP_001124318.1, NP_004199.1, NP_665812.1, NP_665811.1 |
|---|
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Datasheet
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Protocol Information
WB
Western Blot (IB, immunoblot)
IF
Immunofluorescence
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
AIFM1 Antibody (orb1248086)
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