AKT phospho T308 Antibody Biotin Conjugated

SKU: orb344542

Description

AKT pT308 antibody (Biotin)

Images & Validation

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Item 1 of 3

Tested Applications	DOT, ELISA, IHC, WB
Dilution Range	ELISA: 1:20,000, IHC: 20 µg/ml, WB: 1:500 - 1:3,000
Reactivity	Human, Monkey, Mouse, Rat
Application Notes	Biotin Conjugated Anti-AKT pT308 is tested for ELISA, immunohistochemistry, immunoprecipitation and western blotting. Expect a band approximately 56 kDa in size corresponding to phosphorylated AKT protein by western blotting in the appropriate cell lysate or extract. This phospho-specific monoclonal antibody reacts with human and mouse AKT pT308 and shows minimal reactivity by ELISA against the non-phosphorylated form of the immunizing peptide. Specific conditions for reactivity should be optimized by the end user. Use formalin-fixed paraffin-embedded sections for immunohistochemistry. No pre-treatment of sample is required. Read more...

Key Properties

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Antibody Type	Primary Antibody
Host	Mouse
Clonality	Monoclonal
Isotype	IgG1
Clone No.	18F3.H11
Immunogen	Anti-AKT pT308 monoclonal antibody was produced by repeated immunizations with a synthetic peptide corresponding to residues surrounding T308 of human AKT1 protein.
Purity	Anti-AKT pT308 was purified from concentrated tissue culture supernate by Protein A chromatography. This antibody is specific for human and mouse AKT protein phosphorylated at T308. A BLAST analysis was used to suggest cross-reactivity with AKT pT308 from most vertebrate species sources based on 100% homology with the immunizing sequence. Cross-reactivity with AKT from other sources has not been determined. Cross-reactivity with AKT2 and AKT3 will likely occur. Cancer, Cell Signaling, Neuroscience, Signal Transduction research.
Conjugation	Biotin

Storage & Handling

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Storage	Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Form/Appearance	Lyophilized
Buffer/Preservatives	Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: 10 mg/mL Bovine Serum Albumin (rAlbumin) - Immunoglobulin and Protease free; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Concentration	1.0 mg/mL
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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mouse anti-AKT pT308 Biotin conjugated Antibody, Biotin conjugated mouse anti-AKT pT308 Antibody, RAC-PK-alpha, Protein kinase B, PKB, C-AKT, RAC-alpha serine/threonine-protein kinase, Proto-oncogene c-Akt, AKT1, AKT 1, AKT-1, Akt phospho T308 Antibody, Anti-AKT pT308 Monoclonal Antibody Biotin Conjugated

Phospho-AKT1 + AKT2 + AKT3 (Thr308+Thr309+Thr305) Rabbit Polyclonal Antibody (Biotin) [orb501280]

Applications:	FC, ICC, IF, IHC-Fr, IHC-P, WB
Predicted Reactivity:	Bovine, Canine, Gallus, Porcine, Rabbit, Sheep
Reactivity:	Human, Mouse, Rat
Species/Host:	Rabbit
Clonality:	Polyclonal
Conjugation:	Biotin

Sizes Available: 100 μl

AKT1 (Ser473) Recombinant Rabbit mAb, Biotin conjugated [orb1585547]
Applications:
WB
Predicted Reactivity:
Mouse, Rat
Reactivity:
Human
Species/Host:
Rabbit
Clonality:
Recombinant
Conjugation:
Biotin
Sizes Available: 100 μl

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AKT phospho T308 Antibody Biotin Conjugated

ELISA of Mouse anti-Akt phospho T308 Biotin Conjugated antibody. Antigen: Unconjugated Akt phospho T308 and AKT non-phospho T308. Coating amount: 0.1 µg per well. Primary antibody: Akt phospho T308 Biotin Conjugated antibody at 5 µg/ml. Dilution series: 3-fold. Mid-point concentration: 5 ng/mL Akt phospho T308 Biotin Conjugated antibody. Secondary antibody: Peroxidase streptavidin secondary antibody at 1:10000. Substrate: TMB.

Immunohistochemistry of Mouse Anti-AKT phospho T308 Biotin Conjugated. Tissue: prostate at 20X (left) and 40X (right). Fixation: FFPE buffered formalin 10% conc. Antigen retrieval: Heat, Citrate pH6.2. Pressure Cooker, Heat, EDTA pH9.5 Pressure Cooker. Primary antibody: AKT pT308 biotin at 20 µg/ml for 1 h at RT. Secondary antibody: Streptavidin Conj. HRP at 10 ug/ml. Localization: nuclear and occasionally cytoplasmic. Staining: antibody as precipitated red signal with a hematoxylin purple nuclear counterstain.

Western Blot of Mouse anti-Akt phospho T308 Biotin Conjugated antibody. Lane 1: GST tagged AKT1 active recombinant protein. Lane 2: GST tagged AKT1 un-active recombinant protein. Load: 25 ng per lane. Primary antibody: Akt phospho T308 Biotin Conjugated antibody at 1:1000 for overnight at 4°C. Secondary antibody: HRP Streptavidin secondary antibody at 1:40000 for 30 min at RT. Block: orb348637 for 30 min at RT. Predicted/Observed size: 79 kDa, 79 kDa for Akt phospho T308. Other band(s): none

Quick Database Links

UniProt

P31749

RefSeq:62241011

UniProt Details

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No UniProt data available

NCBI Reference Sequences

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Associated Accession Numbers

Curated reference sequences for the gene transcript and protein product

RefSeq	62241011

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Protocol Information

Western Blot (IB, immunoblot)

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IHC

Immunohistochemistry

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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DOT

Dot Blot

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