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ANP32B Recombinant Mouse Monoclonal Antibody

SKU: orb1173067

Description

ANP32B Recombinant Mouse Monoclonal Antibody

Research Area

Apoptosis, Cell Biology, Cell Cycle

Images & Validation

Tested ApplicationsICC, WB
Dilution rangeWB=1:500-1000, ICC/IF=1:100-500
ReactivityHuman
Predicted ReactivityHuman

Key Properties

Antibody TypePrimary Antibody
HostMouse
ClonalityRecombinant
IsotypeIgG2a, Kappa
Clone No.E10F9
TargetANP32B
PurificationAffinity purified by Protein G
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/AppearanceLiquid
Buffer/Preservatives0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Concentration1mg/ml
DisclaimerFor research use only
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ANP32B Recombinant Mouse Monoclonal Antibody

Blocking buffer: 5% NFDM/TBST, Primary ab Dilution: 1:1000, Primary ab incubation condition: 4°C overnight, Lysate: HeLa, MCF-7, JurKat, Protein loading quantity: 20 µg, Exposure time: 30 s, Predicted MW: 29 kDa, Observed MW: 29 kDa.

ANP32B Recombinant Mouse Monoclonal Antibody

Cell line: HepG2, Fixative: 4% Paraformaldehyde, Permeabilization: 0.1% TritonX-100, Primary ab Dilution: 1:50, Primary incubation condition: 4°C overnight Nuclear counter stain: DAPI (Blue), Comment: Color green is the positive signal for orb1173067.

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Protocol Information

WB
Western Blot (IB, immunoblot)
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ICC
Immunocytochemistry
View Protocol

ANP32B Recombinant Mouse Monoclonal Antibody (orb1173067)

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25 μl
$ 180.00
50 μl
$ 330.00
100 μl
$ 490.00
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