AKT (Phospho-S473) Rabbit Monoclonal Antibody

SKU: orb1474576

Description

The AKT (Phospho-S473) Antibody is suitable for WB. It is a Monoclonal, Unconjugated antibody which raised against KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding S473 of human AKT protein. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography.

Images & Validation

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Item 1 of 1

Tested Applications	IF, IHC, WB
Dilution Range	WB (1/500 - 1/1000), IH (1/50 - 1/200), IF/IC (1/50 - 1/200)
Reactivity	Human, Mouse, Rat

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Monoclonal
Immunogen	KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding S473 of human AKT protein. The exact sequence is proprietary.
Target	AKT1
Purification	The antibody was purified by immunogen affinity chromatography.
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 50% Glycerol, 0.01% Sodium azide and 0.05% rAlbumin.
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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PKB; RAC; RAC-alpha serine/threonine-protein kinase; Protein kinase B; PKB; Protein kinase B alpha; PKB alpha; Proto-oncogene c-Akt; RAC-PK-alpha

Quality Guarantee

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AKT (Phospho-S473) Rabbit Monoclonal Antibody

Western blot analysis of AKT (Phospho-S473) expression in C6 (A), NIH3T3 (B), Hela (C) whole cell lysates. (Predicted band size: 56 kD; Observed band size: 56 kD)

Quick Database Links

Gene Symbol

AKT1

UniProt

P31749, P31750, P47196

NCBI Gene ID

24185, 207, 11651

UniProt Details

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No UniProt data available

NCBI Gene Details

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No NCBI Gene data available

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Protocol Information

Western Blot (IB, immunoblot)

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IHC

Immunohistochemistry

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Immunofluorescence

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