B Raf/BRAF Rabbit Polyclonal Antibody

SKU: orb11650

Description

Anti-B Raf/BRAF Antibody. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.

Research Area

Cancer Biology, Cell Biology, Epigenetics & Chromatin, Metabolism Research, Microbiology, Signal Transduction

Images & Validation

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Item 1 of 1

Tested Applications	WB
Dilution Range	Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat Flow Cytometry(Fixed), 1-3 μg/1x10^6 cells, Human
Reactivity	Human, Mouse, Rat

Related Conjugates & Formulations

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Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	Rabbit IgG
Immunogen	A synthetic peptide corresponding to a sequence in the middle region of human B Raf, identical to the related rat and mouse sequences.
Target	Serine/threonine-protein kinase B-raf
Molecular Weight	84 kDa
Purification	Immunogen affinity purified.
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Lyophilized
Buffer/Preservatives	Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
Concentration	Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Disclaimer	For research use only

Alternative Names

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Serine/threonine-protein kinase B-raf; 2.7.11.1; Proto-oncogene B-Raf; p94; v-Raf murine sarcoma viral oncogene homolog B1; BRAF; BRAF1, RAFB1

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Western blot analysis of Lane 1: Rat Brain Tissue Lysate, Lane 2: Rat Testis Tissue Lysate, Lane 3: Rat Liver Tissue Lysate, Lane 4: SW620 Cell Lysate, Lane 5: COLO320 Cell Lysate, Lane 6: PC-12 Cell Lysate using BRAF antibody

Quick Database Links

Gene Symbol

Serine/threonine-protein kinase B-raf

UniProt

P15056

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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