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B7-1 Antibody
SKU: orb1174059
Featured
Description
Images & Validation
−Item 1 of 3
| Tested Applications | ELISA, FC |
|---|---|
| Dilution range | ELISA 1/5000-10000|Flow Cyt 1/100 |
| Reactivity | Human |
| Application Notes |
Key Properties
−| Antibody Type | Recombinant Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Monoclonal |
| Isotype | Rabbit IgG |
| Clone No. | DM109 |
| Target | B7-1 |
| Purification | Purified from cell culture supernatant by affinity chromatography |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Disclaimer | For research use only |
Alternative Names
−CD80,B7,B7-1,B7.1,BB1,CD28LG,CD28LG1,LAB7
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ELISA plate pre-coated by 2 μg/ml (100 μL/well) Human B7-1 protein, hFc tagged protein orb689457 can bind Rabbit anti-B7-1 monoclonal antibody (clone: DM109) in a linear range of 0.6-80 ng/ml.
Flow cytometry analysis with Anti-B7-1 on Expi293 cells transfected with human B7-1 (Blue histogram) or Expi293 transfected with irrelevant protein (Red histogram).
Flow cytometry analysis of antigen binding of rabbit anti-human B7-1 mAb (orb1174059). (A) orb1174059 does not bind to CHO-S cells that do not express B7-1. (B) A clear peak shift of orb1174059 was seen compared to the control when incubated with B7-1-expressing Raji cells, indicating strong binding of orb1174059 to B7-1. Antibodies were incubated at 5 μg/mL.
Quick Database Links
Gene Symbol
B7-1
UniProt
UniProt Details
− No UniProt data available
Documents Download
Datasheet
Product Information
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Protocol Information
FC
Flow Cytometry
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
B7-1 Antibody (orb1174059)
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