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BIK (pS35) Antibody
SKU: orb2649899
Description
Images & Validation
−Item 1 of 3
| Tested Applications | IHC, WB |
|---|---|
| Dilution range | WB: WB (1/500 - 1/1000), IH (1/50 - 1/100), E: WB (1/500 - 1/1000), IH (1/50 - 1/100) |
| Reactivity | Human, Mouse, Rat |
Key Properties
−| Host | Rabbit |
|---|---|
| Clonality | Polyclonal |
| Clone No. | BIK |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Disclaimer | For research use only |
Alternative Names
−NBK; Bcl-2-interacting killer; Apoptosis inducer NBK; BIP1; BP4
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Western blot analysis of BIK (pS35) expression in mouse embryo (A), rat ovary (B), HEK293T-EGF (C), HEK293T (D) whole cell lysates.
Direct ELISA antibody dose-response curve using Anti-BIK (pS35) Antibody. Antigen (phosphopeptide and non-phosphopeptide) concentration is 5 ug/ml. Goat Anti-Rabbit IgG (H&L) - HRP was used as the secondary antibody, and signal was developed by TMB substrate.
Immunohistochemical analysis of BIK (pS35) staining in human lymph node formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
BIK (pS35) Antibody (orb2649899)
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