BRK (Phospho-Y447) Rabbit Polyclonal Antibody

SKU: orb515495

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Description

The BRK (Phospho-Y447) Antibody is suitable for IF, WB. It is a Polyclonal, Unconjugated antibody which raised against KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding Y447 of human BRK protein. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography.

Research Area

Epigenetics

Images & Validation

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Tested Applications	IF, WB
Dilution Range	WB: 1:500-1000, IF/ICC: 1:100-500
Reactivity	Human, Rat

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Immunogen	KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding Y447 of human BRK protein. The exact sequence is proprietary.
Target	PTK6
Purification	The antibody was purified by immunogen affinity chromatography.
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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BRK; Protein-tyrosine kinase 6; Breast tumor kinase; Tyrosine-protein kinase BRK

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Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at support@biorbyt.com.

BRK (Phospho-Y447) Rabbit Polyclonal Antibody

Western blot analysis of BRK (Phospho-Y447) expression in HEK293T (A), rat kidney (B), rat lung (C) whole cell lysates. (Predicted band size: 51 kD; Observed band size: 52 kD)

Immunofluorescent analysis of BRK (Phospho-Y447) staining in NIH3T3 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with Alexa Fluor 647-conjugated secondary antibody (red) in PBS at room temperature in the dark.

Quick Database Links

Gene Symbol

PTK6

UniProt

Q13882

NCBI Gene ID

5753

UniProt Details

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No UniProt data available

NCBI Gene Details

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No NCBI Gene data available

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Protocol Information

Western Blot (IB, immunoblot)

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Immunofluorescence

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