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Anti-CD10 [FR4D11]
Description
Research Area
Images & Validation
−| Tested Applications | FC, IF, IHC |
|---|---|
| Reactivity | Human |
Key Properties
−| Host | Mouse |
|---|---|
| Clonality | Monoclonal |
| Isotype | Mouse IgG |
| Clone No. | FR4D11 |
| Immunogen | This antibody was raised by immunizing mice with Raji cells. |
| Target | CD10 |
| Purity | Purified |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | PBS with 0.02% Proclin 300. |
| Concentration | 1 mg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
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![Anti-CD10 [FR4D11]](/images/pub/media/catalog/product/NewWebsite/35/orb758915_1.png)
Immunofluorescence staining of fixed Daudi cells with anti-CD10 antibody FR4D11. Immunofluorescence analysis of paraformaldehyde fixed Daudi cells on Shi-fix™ coverslips stained with the chimeric rabbit IgG version of FR4D11 (orb758915) at 10 µg/ml for 1h followed by Alexa Fluor® 488 secondary antibody (2 µg/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom orb758915, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody (orb256458) followed by staining with Alexa Fluor® 488 secondary antibody.
![Anti-CD10 [FR4D11]](/images/pub/media/catalog/product/NewWebsite/35/orb758915_2.png)
Western Blot using anti-CD10 antibody FR4D11 Daudi cell lysate (35 µg protein in RIPA buffer) was resolved on a SDS PAGE gel and blots were probed with the chimeric rabbit version of FR4D11 (orb758915) at 2 µg/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.
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Anti-CD10 [FR4D11] (orb758915)
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