Anti-CD23 [CAT-13.9C1]

SKU: orb758911

Description

Rabbit monoclonal antibody to CD23

Research Area

Cancer Biology, Immunology & Inflammation

Images & Validation

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Item 1 of 2

Tested Applications	IF
Reactivity	Human

Key Properties

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Host	Mouse
Clonality	Monoclonal
Isotype	Mouse IgG
Clone No.	CAT-13.9C1
Immunogen	This antibody was raised by immunising mice with with an EBV-transformed human B cell line from a patient with MHC class II deficiency re-expressing class II antigens.
Target	CD23
Purity	Purified
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	PBS with 0.02% Proclin 300.
Concentration	1 mg/ml
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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Low affinity IgE Receptor; Low affinity immunoglobulin epsilon Fc receptor; BLAST-2; C-type lectin domain family 4 member J; Fc-epsilon-RII; Immunoglobulin E-binding factor; Lymphocyte IgE receptor; CAT 13.9C1

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Flow cytometry using the Anti-CD23 antibody CAT-13.9C1. Paraformaldehyde fixed Daudi cells were stained with anti-unknown specificity antibody (orb256458; isotype control, black line) or the rabbit IgG version of CAT-13.9C1 (orb758911, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.

Immunofluorescence staining of fixed Daudi cells with anti-CD23 antibody CAT-13.9C1. Immunofluorescence analysis of paraformaldehyde fixed Daudi cells on Shi-fix™ coverslips stained with the chimeric rabbit IgG version CAT-13.9C1 (orb758911) at 10 µg/ml for 1h followed by Alexa Fluor® 488 secondary antibody (2 µg/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom orb758911, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody (orb256449) followed by staining with Alexa Fluor® 488 secondary antibody.

Quick Database Links

Gene Symbol

CD23

UniProt

P06734

UniProt Details

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No UniProt data available

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Protocol Information

Immunofluorescence

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