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CD4 (Phospho-S433) Rabbit Polyclonal Antibody
SKU: orb1289874
Description
Research Area
Epigenetics, Immunology, Infectious Diseases
Images & Validation
−Item 1 of 3
| Tested Applications | IF, WB |
|---|---|
| Dilution Range | WB: 1:500-1:1000, IH: 1:50-1:200, IF:IC: 1:50-1:200 |
| Reactivity | Canine, Human, Monkey, Mouse, Rat |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Immunogen | KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding S433 of human CD4 protein. The exact sequence is proprietary. |
| Target | CD4 |
| Purification | The antibody was purified by immunogen affinity chromatography. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−T-cell surface glycoprotein CD4; T-cell surface antigen T4/Leu-3; CD4
Quality Guarantee
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Western blot analysis of CD4 (Phospho-S433) expression in mouse spleen (A), rat spleen (B) whole cell lysates. (Predicted band size: 51 kD; Observed band size: 56 kD)
Immunofluorescent analysis of CD4 (Phospho-S433) staining in HepG2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a Alexa Fluor 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
Direct ELISA antibody dose-response curve using Anti-CD4 (Phospho-S433) Antibody. Antigen (Phosphopeptide and non-phosphopeptide) concentration is 5 ug/ml. Goat Anti-Rabbit IgG (H&L) - HRP was used as the secondary antibody, and signal was developed by TMB substrate.
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Protocol Information
WB
Western Blot (IB, immunoblot)
IF
Immunofluorescence
CD4 (Phospho-S433) Rabbit Polyclonal Antibody (orb1289874)
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