Anti-CD40L [AT161-10]

SKU: orb758776

Description

Rabbit monoclonal antibody to CD40L

Images & Validation

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Item 1 of 2

Tested Applications	ELISA
Reactivity	Human

Key Properties

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Host	Mouse
Clonality	Monoclonal
Isotype	Mouse IgG
Clone No.	AT161-10
Immunogen	This antibody was raised by immunising mice with Chinese hamster ovary (CHO) cells expressing human CD40L.
Target	CD40L
Purity	Purified
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	PBS with 0.02% Proclin 300.
Concentration	1 mg/ml
Disclaimer	For research use only

Alternative Names

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CD154; CD40 ligand; CD-40L; CD-40 Ligand; CD40-L; CD-40-L

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Flow-cytometry using the anti-CD40L antibody AT161-10. Human peripheral blood leukocytes were stained with anti-Fluorescein IgG antibody (4-4-20; isotype control, black line) or the rabbit IgG1 version of AT161-10 (orb758776, blue line) at a dilution of 1:100 for 1h at RT. After washing, bound antibody was detected using a goat anti-mouse IgG AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.

Immunofluorescence staining of human peripheral blood monocytes using anti-CD40L antibody AT161-10. Immunofluorescence analysis of paraformaldehyde fixed human peripheral blood monocytes immobilized on Shi-fix™ cover-slips and stained with the chimeric rabbit IgG version of AT161-10 (orb758776) at 10 µg/ml followed by Alexa Fluor® 488 secondary antibody (2 µg/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom orb758776, DAPI, merged channels and an isotype control. The isotype control was stained with anti-Fluorescein antibody (orb256390) followed by Alexa Fluor® 488 secondary antibody.

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Gene Symbol

CD40L

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Protocol Information

ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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