Anti-CD40L [hu5c8 (Ruplizumab)]

SKU: orb348859

Description

Human monoclonal antibody to CD40L

Images & Validation

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Item 1 of 2

Tested Applications	Blocking, FC, WB
Reactivity	Human

Key Properties

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Host	Human
Clonality	Monoclonal
Isotype	IgG1
Clone No.	hu5c8 (Ruplizumab)
Immunogen	Human CD40L
Target	CD40L
Purity	Purified
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	PBS with 0.02% Proclin 300.
Concentration	1 mg/ml
Disclaimer	For research use only

Alternative Names

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CD154; CD40 ligand; tumour necrosis factor ligand superfamily member 5; TNFSF5; tumour necrosis factor related activation protein; TRAP

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Flow-cytometry using the anti-CD40L research biosimilar antibody Ruplizumab (hu5c8) Human lymphocytes were stained with an isotype control (orb256390, panel A) or the rabbit-chimeric version of Ruplizimab (orb348886, panel B) at a concentration of 1 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody and cells analysed on a FACSCanto flow-cytometer.

Western Blot using anti-OX40L antibody. Rat spleen (lane 1), rat muscle (lane 2), rat bladder (lane 3), human breast tumour (lane 4) and human thyroid tumour (lane 5) samples were resolved on a 10% SDS PAGE gel and blots probed with orb348859 at 1 µg/ml before being detected by a secondary antibody. The expected band size (29 kDa) is seen in the human-derived samples, as well as bands corresponding to dimers and other multimers.

Quick Database Links

Gene Symbol

CD40L

UniProt

P29965

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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Flow Cytometry

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