GPC3/Glypican-3 Antibody (MMAE)

SKU: orb1805490

Description

Anti-GPC3 / Glypican-3 Reference Antibodyd Monomethyl auristatin E (MMAE) used for the study of hepatocellular carcinoma.

Images & Validation

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Item 1 of 4

Tested Applications	ELISA, FA, FACS, In vivo
Reactivity	Human

Key Properties

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Antibody Type	Recombinant Antibody
Clonality	Recombinant
Target	GPC3 / Glypican-3
Source/Expression System	CHO
Molecular Weight	150.85 kDa
Purity	>95%
Purification	Protein A
Endotoxin	<0.001 EU/ug
Conjugation	MMAE

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	100 mM Proline/Acetic acid 20mM Arginine pH 5.0
Concentration	Batch dependent
Disclaimer	For research use only

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Anti-GPC3 / Glypican-3 Reference Antibody (Codrituzumab-MMAE) on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.

The purity of Anti-GPC3 / Glypican-3 Reference Antibody (Codrituzumab-MMAE)is more than 99.73% ;determined by SEC-HPLC.

The killing ratio Codrituzumab-MMAE by HuH-7 increased with the increase of antibody concentration; and the killing Rate (%) reached 130% at antibody concentration of 40 nM.

Quick Database Links

Gene Symbol

GPC3 / Glypican-3

UniProt

P51654

UniProt Details

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No UniProt data available

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Protocol Information

FACS

Fluorescence-Activated Cell Sorting (FC, Flow cytometry)

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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