Histone H4 (Phospho-S47) Rabbit Polyclonal Antibody

SKU: orb224030

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Description

The Histone H4 (Phospho-S47) Antibody is suitable for IF, WB. It is a Polyclonal, Unconjugated antibody which raised against KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding S47 of human Histone H4 protein. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography.

Research Area

Epigenetics

Images & Validation

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Tested Applications	IF, WB
Dilution Range	WB: 1:500-1:1000, IF/ICC: 1:100-1:500
Reactivity	Human, Rat

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Immunogen	KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding S47 of human Histone H4 protein. The exact sequence is proprietary.
Target	HIST1H4A
Purification	The antibody was purified by immunogen affinity chromatography.
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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H4/A; H4FA; H4/I; H4FI; H4/G; H4FG; H4/B; H4FB; H4/J; H4FJ; H4/C; H4FC; H4/H; H4FH; H4/M; H4FM; H4/E; H4FE; H4/D; H4FD; H4/K; H4FK; H4/N; H4F2; H4FN; HIST2H4; H4/O; H4FO; Histone H4

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Histone H4 (Phospho-S47) Rabbit Polyclonal Antibody

Western blot analysis of Histone H4 (Phospho-S47) expression in HEK293T (A), U2OS (B) whole cell lysates. (Predicted band size: 11 kD; Observed band size: 11 kD)

Immunofluorescent analysis of Histone H4 (Phospho-S47) staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

Quick Database Links

Gene Symbol

HIST1H4A

UniProt

P62805

NCBI Gene ID

8359, 121504, 8363, 8362, 8365, 8367, 8361, 554313, 8364, 8360, 8294, 8366, 8370, 8368

UniProt Details

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No UniProt data available

NCBI Gene Details

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No NCBI Gene data available

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Protocol Information

Western Blot (IB, immunoblot)

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Immunofluorescence

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