Tenascin C Antibody

Separation of U-87 MG cells stained using anti-tenascin C (T2H5) purified antibody (concentration in sample 12 µg/ml, GAM FITC, red-filled) from U-87 MG cells unstained by primary antibody (GAM FITC, black-dashed) in flow cytometry analysis (surface staining).

Immunocytochemistry staining of tenascin C in U-87 MG cells using purified mouse monoclonal antibody T2H7 (concentration in sample 12 µg/ml, GAM FITC, right picture) vs. Hoechst 34580 nuclear staining (left picture).

Immunohistochemistry staining of tonsil (paraffin-embedded sections) with anti-human tenascin C (T2H5).

Western blotting analysis of human tenascin C using mouse monoclonal antibody T2H5 on lysates of U87-MG cell line and Jurkat cell line (tenascin non-expressing cell line; negative control) under non-reducing and reducing conditions. Cells were lysed by 50 mM TRIS-Cl pH 6.6, 4M urea, 4% SDS, samples were mixed and heated (100°C, 5 min) with reducing and non-reducing SDS-loading buffer, then resolved using 7.5% Tris-glycine SDS gel electrophoresis. Nitrocellulose membrane was probed with 2 µg/ml of mouse anti-tenascin monoclonal antibody followed by IRDye800-conjugated anti-mouse secondary antibody. Tenascin C was detected slightly above 250 kDa.