Anti-IL-12/23 [ABT-874 (Briakinumab)]

SKU: orb2645663

Description

Research Area

Immunology & Inflammation

Images & Validation

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Item 1 of 2

Tested Applications	Blocking
Reactivity	Human

Key Properties

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Host	Human
Clonality	Monoclonal
Isotype	IgG1
Clone No.	ABT-874 (Briakinumab)
Immunogen	This antibody was generated by phage display technology.
Target	IL-12/23
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	PBS with 0.02% Proclin 300.
Concentration	1 mg/ml
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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IL-12p40; Interleukin 12; Interleukin 23; IL12; IL23; IL-12; IL-23

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Flow cytometry using anti-IL-12/2 antibody ABT-874 (Briakinumab). Paraformaldehyde-fixed Jurkat cells permeabilized with 0.5% Triton were stained with the anti-unknown specificity antibody (orb256458; isotype control, black line) or the rabbit IgG version of ABT-874 (orb2645662, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor® 488 antibody at a dilution of 1:1000, and the cells were analyzed using a FACSCanto flow-cytometer.

Immunofluorescence staining of Jurkat cells with anti-IL-12/23 antibody ABT-874 (Briakinumab). Immunofluorescence analysis of paraformaldehyde fixed Jurkat cells on Shi-fix™ coverslips stained with the chimeric rabbit IgG version of ABT-874 (Briakinumab) (orb2645662) (1:100 dilution) for 1h followed by Alexa Fluor® 488 secondary antibody (1:1000 dilution), showing membrane and cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show, from left-right, top-bottom, orb2645662, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody (orb256458) followed by staining with Alexa Fluor® 488 secondary antibody.

Quick Database Links

Gene Symbol

IL-12/23

UniProt

P29460

UniProt Details

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No UniProt data available

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