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IL-21/Il21 Antibody
SKU: orb692190
Description
Images & Validation
−Item 1 of 2
| Tested Applications | ELISA, IHC |
|---|---|
| Reactivity | Mouse, Rat |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Immunogen | E.coli-derived mouse IL-21/Il21 recombinant protein (Position: H18-S146). |
| Target | Neutrophil gelatinase-associated lipocalin |
| Molecular Weight | 65-80 kDa |
| Purification | Immunogen affinity purified. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Lyophilized |
| Concentration | Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml. |
| Disclaimer | For research use only |
Alternative Names
−Beta-2-microglobulin; B2m
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IHC analysis of IL-21/Il21 using anti-IL-21/Il21 antibody. IL-21/Il21 was detected in paraffin-embedded section of mouse spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 µg/ml rabbit anti-IL-21/Il21 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
IHC analysis of IL-21/Il21 using anti-IL-21/Il21 antibody. IL-21/Il21 was detected in paraffin-embedded section of rat spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 µg/ml rabbit anti-IL-21/Il21 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
Quick Database Links
Gene Symbol
Neutrophil gelatinase-associated lipocalin
UniProt
UniProt Details
− No UniProt data available
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Protocol Information
IHC
Immunohistochemistry
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
IL-21/Il21 Antibody (orb692190)
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