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Anti-IL-5 [2E3]
SKU: orb669750
Description
Research Area
Cancer Biology, Immunology & Inflammation
Images & Validation
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| Tested Applications | ELISA |
|---|---|
| Reactivity | Human |
Key Properties
−| Host | Mouse |
|---|---|
| Clonality | Monoclonal |
| Isotype | Mouse IgG |
| Clone No. | 2E3 |
| Immunogen | This antibody was raised by immunising CAF1 mice with human recombinant IL-5. |
| Target | IL-5 |
| Purity | Purified |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | PBS with 0.02% Proclin 300. |
| Concentration | 1 mg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−interleukin 5; IL5; IL 5; Eosinophil differentiation factor
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Flow-cytometry using the Anti-IL-5 antibody 2E3. Human peripheral blood leukocytes were rested for 4 hours. The cells were fixed using 2% PFA, permeabilized using 0.5% Triton and stained with anti-Fluorescein IgG antibody (4-4-20; isotype control, black line) or the rabbit IgG version of 2E3 (orb669750, blue line) at a dilution of 1:100 for 1h at RT. After washing, bound antibody was detected using a goat anti-mouse IgG AlexaFluor® 488 antibody at a dilution of 1:1000 and cells were analyzed using a FACSCanto flow-cytometer.
Quick Database Links
Gene Symbol
IL-5
UniProt
UniProt Details
− No UniProt data available
Documents Download
Datasheet
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Anti-IL-5 [2E3] (orb669750)
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