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LCK (pY393) Antibody
SKU: orb2651391
Description
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−Item 1 of 3
| Tested Applications | IF, WB |
|---|---|
| Dilution range | WB: WB (1/500 - 1/1000), IF/IC (1/100 - 1/500), IF: WB (1/500 - 1/1000), IF/IC (1/100 - 1/500), E: WB (1/500 - 1/1000), IF/IC (1/100 - 1/500) |
| Reactivity | Human, Mouse, Porcine, Rat, Virus |
Key Properties
−| Host | Rabbit |
|---|---|
| Clonality | Polyclonal |
| Clone No. | LCK |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Disclaimer | For research use only |
Alternative Names
−Tyrosine-protein kinase Lck; Leukocyte C-terminal Src kinase; LSK; Lymphocyte cell-specific protein-tyrosine kinase; Protein YT16; Proto-oncogene Lck; T cell-specific protein-tyrosine kinase; p56-LCK
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Western blot analysis of LCK (pY393) expression in Myla2059 (A), HuT78 (B) whole cell lysates.
Direct ELISA antibody dose-response curve using Anti-LCK (pY393) Antibody. Antigen (phosphopeptide and non-phosphopeptide) concentration is 5 ug/ml. Goat Anti-Rabbit IgG (H&L) - HRP was used as the secondary antibody, and signal was developed by TMB substrate.
Immunofluorescent analysis of LCK (pY393) staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4°C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
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Protocol Information
WB
Western Blot (IB, immunoblot)
IF
Immunofluorescence
LCK (pY393) Antibody (orb2651391)
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